Medical Entomology and Zoology (2010) 61, 335-344

Mitsuhiro Iwasa and Naotaka Ishiguro (2010)
Genetic and morphological differences of Haematobia irritans and H. exigua, and molecular phylogeny of Japanese Stomoxyini flies (Diptera, Muscidae)
Medical Entomology and Zoology 61 (4), 335-344
Abstract: Molecular analysis based on mitochondrial DNA (mtDNA) sequence was performed to elucidate the genetic difference between Haematobia irritans (Linnaeus) and H. exigua (de Meijere), and the phylogenetic relationship among Japanese Stomoxyini species (Stomoxys calcitrans (Linnaeus), S. indicus Picard, S. uruma Shinonaga et Kano, and Haematobosca sanguinolenta (Austen) including Haematobia). Two populations obtained from Obihiro and Morioka which were morphologically identified as H. irritans were genetically identical. Two populations obtained from Taiwan and Vietnam which were morphologically identified as H. exigua were almost genetically identical, but there was a small divergence (0.3-0.4%) between them. Nucleotide sequence divergences between H. irritans from Obihiro-Morioka and H. exigua from Taiwan-Vietnam were 1.8-1.9%, and those between Haematobia and other separate species of Stomoxys and Haematobosca were 6.7-10.9%. In cluster analysis based on mtDNA, Haematobia species were close to Haematobosca sanguinolenta. S. calcitrans and S. uruma were closely related to each other, and S. indicus was distantly related to these two species. We discussed the status of H. irritans and H. exigua based on mtDNA, and the discrepancies of molecular phylogeny versus the previous hypotheses based on morphological criteria in Japanese Stomoxyini.
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Research topic(s) for pests/diseases/weeds:
general biology - morphology - evolution
molecular biology - genes

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Musca domestica		Japan
	Stomoxys calcitrans		Japan
	Haematobia irritans		Japan
	Haematobia exigua		Taiwan
	Haematobia exigua		Vietnam
	Stomoxys indicus		Japan